30 th Southern Tree Improvement Conference

نویسندگان

  • Tom Byram
  • Barbara Crane
  • Jeff Dean
  • Ulrika Egertsdotter
  • Barry Flinn
  • Fikret Isik
  • Steve McKeand
چکیده

Glutathione/ glutathione disulfide and ascorbic acid /dehydroascorbateare two major redox pairs that control the redox-state in a developing seed.Recently these redox compounds have been shown to exert strong positive effectson embryo development in several plants including white spruce. A picture isemerging that early-stage embryo development occurs best in the presence of areducing environment while late-stage development requires a shift to anoxidizing environment. Knowledge of physiological concentrations of thesecompounds during pine embryo development would allow us to better mimic theseed environment and improve somatic embryo development. Triplicate analysis for the seed redox chemicals ascorbic acid (AsA),dehydroascorbate (DHA), glutathione (GSH) and glutathione disulfide (GSSG)were completed for developmentally staged zygotic embryos and femalegametophytes over the sequence of loblolly pine (Pinus taeda L) seeddevelopment. The two trees tested generally showed similar concentrations andpatterns of change through time. The redox potential changed significantlythrough the progression of seed development. These profiles suggest that early-stage embryo development occurs best in the presence of a reducing environmentwhile late-stage development requires a shift to an oxidizing environment. Theredox chemical profiles obtained provide guidelines and targets to mimic in thetissue culture environment. Recent and ongoing tests have shown that alteration of the in vitro redoxenvironment through medium supplementation with glutathione or glutathionedisulfide during specific developmental stages can indeed enhance loblolly pinesomatic embryo development and germination.

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تاریخ انتشار 2009